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The Importance of Understanding Drivers of Irritation In Vivo for Selection of Chemicals Used in the Development and Evaluation of In Vitro Eye Irritation Assays: Cosmetics Europe Analysis.

Poster presented by the Cosmetics Europe AAT Eye Irritation Task Force at In Vitro Testing Industrial Platform (IVTIP) 2013 meeting, 15-16 May 2013, Southampton, U.K. (http://www.ivtip.org).

Selecting chemicals for use in the development and evaluation of in vitro eye irritation assays is a critical and essential element that enables identification and evaluation of predictive capacity and applicability domain at an early stage of method development. Such a selection needs to be based on a thorough understanding of what drives irritation in classification of ocular effects of chemicals in the in vivo rabbit Draize test. This poster summarises an in depth analysis that was undertaken by Cosmetics Europe’s Task Force Eye Irritation (TFEI) of publically available external databases containing already existing in vivo eye irritation data for chemicals tested in the Draize eye irritation test with the objective to better understand the importance of such drivers of irritation for this purpose.

Poster presented by the Cosmetics Europe AAT Genotoxocity Task Force at the 49th Congress of the European Societies of Toxicology (EUROTOX) 2013 meeting, September 1 to 4, 2013, Interlaken, Switzerland (http://www.eurotox2013.com).

The Cosmetic Directive and REACH have demanded that novel in vitro methods should be developed for the future risk assessment of chemicals for their potential to cause toxicity. The Cosmetics Europe Genotoxicity Taskforce was set up to address the high rate of “false” positive results in vitro clastogenicity assays. This poster summarizes results from three projects which aimed to (a) refine current in vitro assays, (b) investigate alternative methods combining reconstructed 3D skin with the micronucleus and Comet assays, (c) measure and compare the xenobiotic metabolizing capacity of native human skin and RS models. The findings from these projects have helped to improve the predictive capacity of in vitro cytogenetic assays.

The importance of understanding drivers of irritation in vivo for selection of chemicals used in the development and evaluation of in vitro eye irritation assays: Cosmetics Europe analysis.

Poster presented by the Cosmetics Europe AAT Eye Irritation Task Force at the 49th Congress of the European Societies of Toxicology (EUROTOX) 2013 meeting, September 1 to 4, 2013, Interlaken, Switzerland (http://www.eurotox2013.com).

Selecting chemicals for use in the development and evaluation of in vitro eye irritation assays is a critical and essential element that enables identification and evaluation of predictive capacity and applicability domain at an early stage of method development. Such a selection needs to be based on a thorough understanding of what drives irritation in classification of ocular effects of chemicals in the in vivo rabbit Draize test. This poster summarises an in depth analysis that was undertaken by Cosmetics Europe’s Task Force Eye Irritation (TFEI) of publically available external databases containing already existing in vivo eye irritation data for chemicals tested in the Draize eye irritation test with the objective to better understand the importance of such drivers of irritation for this purpose.

Poster presented by the Cosmetics Europe AAT Genotoxicity Task Force at The Society of Toxicology (SOT) 52nd Annual Meeting, 10-14 March 2013, San Antonio, Texas, USA (http://www.toxicology.org/AI/MEET/AM2013/).

This poster summarises the preliminary validation results for the reconstructed 3D human skin micronucleus assay. The assay was shown to be transferable to different laboratories and it can reproducibly and correctly predict the genotoxic potential of true positive, false positive and negative chemicals. The project entered the third phase of validation in which the number of coded chemicals tested was increased to 29. The results are promising and support the use of the reconstructed 3D human skin micronucleus assay to measure the genotoxic potential of dermally applied chemicals.